ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (6): 903-910.doi: 10.11843/j.issn.0366-6964.2015.06.005

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Differences of Early Innate Immunity between Chicken Breeds against Salmonella Enteritidis Infection

LIU Lu1,2,LI Jian-chao1,2,LIU Ran-ran2,LI Qing-he2,WEN Jie2,CHANG Guo-bin1,ZHENG Mai-qing2,CHEN Guo-hong1* ,ZHAO Gui-ping2*   

  1. (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
  • Received:2014-11-07 Online:2015-06-23 Published:2015-06-23

Abstract:

This study was conducted to study the effect of S.Enteritidis infection on early immune performance between two chicken breeds(the Beijing-You chicken(BJY),a Chinese indigenous breed,and the White Leghorn(WL),an imported laying type breed).40 BJY chickens and 40 WL chickens on 1-day-old were reared in isolators during the same period and were challenged by intramuscular injection with 8.7×108 cfu•mL-1 per chick(0.5 mL) of S.Enteritidis suspension,the concentrations of cytokines,antibodies and the S.Enteritidis colonization level in the blood and the expression of TLR4 and methyltransferase genes were determined in each group at 12,24,72 and 144 h after post-infection (12 hpi,24 hpi,72 hpi,144 hpi).The results showed that:(1)After challenged by S.Enteritidis,the two breeds showed significant differences in the serum antibody levels of IgY,IL-6,TNF-α,the antibody levels in BJY were higher(P<0.05);(2) The SE colonization level in the blood of BJY was significantly lower than that of WL at 144 hpi (P<0.05),while there were no differences at 12 hpi,24 hpi,72 hpi between two breeds(P>0.05);(3) At 12 hpi the relative quantity of TLR4 in caecum in WL chickens was higher than that in BJY chickens,while at 24 hpi BJY chickens was significantly higher than that in WL chickens(P<0.05).There were significant differences for methyltransferase genes (DNMT1,DNMT3A and DNMT3B) expression in caecum between the two breeds(P<0.05).The results demonstrated that the two breeds differed markedly in immune traits after S.Enteritidis infection.BJY chicken was superior than WL chickens in considering of immune index.

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